Comparative Study of In Vitro Root and Shoot Proliferation from the Node Explants of Asparagus racemosus Willd

  • K. K. Dahal Central Department of Botany, Tribhuvan University
  • S. D. Joshi Central Department of Botany, Tribhuvan University
Keywords: Asparagus racemosus, in vitro, tissue culture, phyto-hormones, adventitious shoots

Abstract

Asparagus racemosus Willd. locally known as Kurilo or Shatavari is found throughout the tropical and subtropical regions of Nepal. It is a high value herb because of its medicinal and nutritional values which is leading this species decrease in its natural habitat. Hence, to conserve this species, an in vitro tissue culture method of its multiplication has been applied as an effective method of ex situ conservation. In the experiment, although most of the treatments induced highly insignificant number of adventitious shoots, low concentration of IBA (0.1-0.5 mg/l) in combination with relatively higher concentrations of Kinetin (1.0-2.0 mg/l) in MS medium were found to be significantly inducing up to 8.33±1.308 shoots/node. Among the treatments where hormones were used singly, IAA 0.5 and Kinetin 1.0 induced 4.83±1.08 and 4.66±1.43 shots/node respectively. Hence, a protocol for the effective multiplication of this species has been developed which can be used accordingly.

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Abstract
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pdf
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Published
2018-12-03
How to Cite
Dahal, K., & Joshi, S. (2018). Comparative Study of In Vitro Root and Shoot Proliferation from the Node Explants of Asparagus racemosus Willd. Journal of the Institute of Agriculture and Animal Science, 35(1), 121-125. https://doi.org/10.3126/jiaas.v35i1.22523
Section
Research Articles