Analysis of Phytoconstituents and Biological Activities on The Selected Medicinal Plants of Dolakha and Sindhualchowk District of Nepal

Bimala Subba, Susila Thapa

Abstract

Phytochemical and biological activities of methanolic extract of seven plants viz. Scindapsus officinalis Schott. , Lepisorus loriformis, Nicotiana tabacum L., Clematis buchananiana DC., Astilbe rivularis D. Don, Potentilla fulgens Wall. ex Hook and Taxus wallichiana Zucc. were carried out. The brine shrimp bioassay showed that N. tabacum, A. rivularis, and P. fulgens were pharmacologically active. The antibacterial potential was studied against Staphylococcus aureus (gram positive bacteria) and Escherichia coli (gram negative bacteria) using Agar Well Diffusion Method. Roots of P. fulgens showed inhibition against gram positive bacteria while the rhizome of A. rivularis showed inhibition against both gram positive and gram negative bacteria. Antioxidant activity was evaluated by 2, 2-diphenyl-1-picryl hydrazyl (DPPH) free radical scavenging activity and ferric reducing antioxidant power FRAP assay. Both assays showed that P. fulgens has high antioxidant activities with (IC50 =15.57 ± 3.6) lower than standard ascorbic acid.

  Journal of Institute of Science and Technology

Volume 22, Issue 2, January 2018, Page: 140-147

Keywords

Antioxidant activity, Agar well diffusion method, Brine shrimp bioassay, DPPH assay, FRAP assay

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DOI: http://dx.doi.org/10.3126/jist.v22i2.19605

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