STUDY ON THE CHEMICAL CONSTITUENTS AND ANTIBACTERIAL ACTIVITY OF ESSENTIAL OIL OF ACORUS CALAMUS L. RHIZOMES OF RUPENDEHI DISTRICT (NEPAL)

Authors

  • Susan Joshi Central Department of Chemistry, Tribhuvan University, Kirtipur, Kathmandu
  • Sundar Bashyal Central Department of Chemistry, Tribhuvan University, Kirtipur, Kathmandu

DOI:

https://doi.org/10.3126/jist.v23i1.22196

Keywords:

Acorus calamus L., Rhizomes, Essential oil, β-asarone, Isoelemicine, Escherichia coli

Abstract

The essential oil from the air dried powder of wild Acorus calamus L. rhizomes was isolated using Clevenger-type hydrodistillation apparatus and its chemical constituents were analyzed by gas chromatography-mass spectrometric (GC-MS) analysis. A total numbers of fourteen chemical constituents were identified and quantified occupying 100 % of total oil composition. The major chemical constituents are reported to be β-asarone (68.96 %), 4-hydroxy-4-methyl-2-pentanone (8.73 %) shyobunone (8.65 %), p-methoxyphenyl butanone (4.16 %) and the minor chemical constituents are δ-cadinene (0.84 %), isoelemicine (2.68 %), muurolene (1.92 %) and cis-ocimene (1.44 %). The antibacterial activity of the essential oil isolated from the wild Acorus calamus L. rhizomes was carried out using paper disc method against one Gram positive bacteria of Staphylococcus aureus and three Gram negative bacteria of Escherichia coli, Klebsiella pneumoniae and Proteus vulgaris. The isolated essential oil from the dry powder of Acorus calamus L. rhizome showed a good potentiality of the antibacterial effect against tested bacteria.

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Published

2019-01-01

How to Cite

Joshi, S., & Bashyal, S. (2019). STUDY ON THE CHEMICAL CONSTITUENTS AND ANTIBACTERIAL ACTIVITY OF ESSENTIAL OIL OF ACORUS CALAMUS L. RHIZOMES OF RUPENDEHI DISTRICT (NEPAL). Journal of Institute of Science and Technology, 23(1), 57–60. https://doi.org/10.3126/jist.v23i1.22196

Issue

Vol. 23 No. 1 (2018)

Section

Research Articles

License

The views and interpretations in this journal are those of the author(s). They are not attributable to the Institute of Science and Technology, T.U. and do not imply the expression of any opinion concerning the legal status of any country, territory, city, area of its authorities, or concerning the delimitation of its frontiers of boundaries.

The copyright of the articles is held by the Institute of Science and Technology, T.U.