Phytochemical Screening, Biological Studiesand GC-MS Analysis of Extract of Biden pilosa L

  • Dharma Prasad Khanal Manmohan Memorial Institute of health Sciences, Soalteemode, Kathmandu, Nepal
  • Rupa Rana Manmohan Memorial Institute of Health Sciences, Soalteemode, Kathmandu, Nepal
  • Bechan Raut Manmohan Memorial Institute of Health Sciences, Soalteemode, Kathmandu, Nepal
  • Rabindra Prasad Dhakal Nepal Academy of Science and Technology, National Forensic Science Laboratory, Khumaltar, Lalitpur, Nepal
Keywords: Bidens pilosa L, Extract, phytochemicals, biological activity, GC-MS analysis

Abstract

Objective: The aim of the research work was to carry out the extraction of areal parts of Biden pilosa L by hydroalcholic and Hexane, ethyl acetate and acetone mixture followed by qualitative phytochemical analysis, acute oral toxicity test, anti-inflammatory test and GC-MS analysis of the extracts.

Method: The hydro-alcoholic and HEA(n-hexane, ethyl acetate and acetone) extraction was done from aerial parts using ethanol and water in the ratio 70:30 and n-hexane, ethyl acetate and acetone in the ratio of 1:1:1 (HEA extract)  respectively. Acute oral toxicity testwas performed OECD guidelines. The single spot in TLC was obtained using n-hexane as solvent for HEA fraction and finally phytocomponents were identified by GC-MS present in that spot.In vitro anti-inflammatory activity was performed by human RBC membrane stabilization method.

Result: The phytochemical test results obtained indicate that hydro-alcoholic extract of aerial part of Bidens pilosa L. possess alkaloids, tannins, terpenoids and saponins whereas HEA extract possess alkaloids, flavonoids, tannins. Both hydro-alcoholic and HEA extracts were found to be safe up to the dose of 2000 mg/kg BW of the mice. Both extracts showed significant in vitro anti-inflammatory activity in a concentration dependent manner. The GC-MS analysis of HEA extract of aerial parts showed the presence of the sixteen different compounds from partially separated extract from TLC plates.

Conclusion: Hydro-alcoholic extract of aerial part of Bidens pilosa L. possess alkaloids, tannins, terpenoids and saponins whereas HEA extract possess alkaloids, flavonoids, tannins. Both hydro-alcoholic and HEA extracts were found to be safe up to the dose of 2000 mg/kg BW of the mice. The GC-MS analysis of HEA extract of aerial parts showed the presence of the sixteen different compounds.

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Abstract
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Published
2019-05-14
How to Cite
Khanal, D., Rana, R., Raut, B., & Dhakal, R. (2019). Phytochemical Screening, Biological Studiesand GC-MS Analysis of Extract of Biden pilosa L. Journal of Manmohan Memorial Institute of Health Sciences, 5(1), 79-93. https://doi.org/10.3126/jmmihs.v5i1.24076
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