Anti-inflammatory, Anxiolytic and Antioxidant Property of Lactuca sativa L and Formulation of Microspheres Loaded Sustained Release Anti-inflammatory Gel

  • Rajendra Gyawali Kathmandu University, Dhulikhel, Kavre
  • Anupama Ghimire Kathmandu University, Dhulikhel, Kavre
  • Apeksha Khatiwada Kathmandu University, Dhulikhel, Kavre
  • Prakshit Niraula Kathmandu University, Dhulikhel, Kavre
  • Ushma Sharma Kathmandu University, Dhulikhel, Kavre
  • Roshani Thapa Kathmandu University, Dhulikhel, Kavre
Keywords: Lactuca sativa L, anti-inflammatory and anxiolytic activity, antioxidant, microspheres, sustained release gel

Abstract

Anti-inflammatory and anxiolytic properties of Lactuca sativa L extract was evaluated by formalin induced mice paw edema test, and elevated plus maze animal model respectively. Solid microspheres containing dispersed extract were obtained by water-in-oil-in-water multiple emulsion system followed by solvent evaporation method. Optimization was carried out using MINITAB 16 and optimized microspheres were loaded into a gel base. Anti-oxidant activity of optimized microspheres loaded gel was evaluated using DPPH (2,2-diphenyl-1-picrylhydrazyl) assay. The gel was also evaluated for its physical properties, drug release kinetics and anti-inflammatory activity using formalin induced mice-paw edema test where the anti-inflammatory potential was compared to marketed diclofenac gel (1.16% w/w). All doses of extract as well as microsphere loaded sustained release anti-inflammatory gel showed a dose and time dependent inhibition of edema (P<0.05), the extract also showed a dose dependent inhibition of level of anxiety (P<0.001). Potential anti-oxidant activity was seen with different concentration of crude extract and microsphere loaded gel. The anti-oxidant potential was compared using IC50 value which was 5.62 µg/mL, 6.34 µg/ml, 8.25 µg/mL for ascorbic acid, methanolic extract of L. sativa and microspheres loaded gel respectively.

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Author Biographies

Rajendra Gyawali, Kathmandu University, Dhulikhel, Kavre

Department of Pharmacy

Anupama Ghimire, Kathmandu University, Dhulikhel, Kavre

Department of Pharmacy

Apeksha Khatiwada, Kathmandu University, Dhulikhel, Kavre

Department of Pharmacy

Prakshit Niraula, Kathmandu University, Dhulikhel, Kavre

Department of Pharmacy

Ushma Sharma, Kathmandu University, Dhulikhel, Kavre

Department of Pharmacy

Roshani Thapa, Kathmandu University, Dhulikhel, Kavre

Department of Pharmacy

Published
2020-07-29
How to Cite
Gyawali, R., Ghimire, A., Khatiwada, A., Niraula, P., Sharma, U., & Thapa, R. (2020). Anti-inflammatory, Anxiolytic and Antioxidant Property of Lactuca sativa L and Formulation of Microspheres Loaded Sustained Release Anti-inflammatory Gel. Journal of Nepal Chemical Society, 41(1), 8-15. https://doi.org/10.3126/jncs.v41i1.30371
Section
Articles