Synthesis and Characterization of High Molecular Weight Chitosan, and Antioxidant Activity of Its Chitosan Oligosaccharide Encapsulation

Authors

  • Hari Sharan Adhikari Department of Applied Sciences, Pashchimanchal Campus, Institute of Engineering, Tribhuvan University, Pokhara
  • Aditya Garai Department of Inorganic and Physical Chemistry, Indian Institute of Science, Bangalore
  • Bishnu Prasad Marasini Department of Biotechnology, National College, Tribhuvan University, Kathmandu
  • Rameshwar Adhikari Research Center for Applied Science and Technology, Tribhuvan University, Kathmandu
  • Paras Nath Yadav Central Department of Chemistry, Tribhuvan University, Kathmandu

DOI:

https://doi.org/10.3126/jncs.v42i1.35326

Keywords:

Antioxidant activity, chitin, chitosan oligosaccharide, high molecular weight nanocrystalline chitosan, encapsulation

Abstract

High molecular weight nanocrystalline chitosan (HMWNC)with Mw of 350 kDa and 66.92% DDA was prepared by alkaline N-deacetylation of chitin that was abundantly obtained after demineralization and deproteinization of pulverized crab shells, and commercially available chitosan oligosaccharide (COS) with average Mw< 3000 Da and 87% DDA was encapsulated with HMWNC. The encapsulation of COS as a pharmaceutical ingredient into HMWC was hypothesized to enhance the bioavailability of COS in target cells. The HMWNC encapsulated chitosan oligosaccharide (COS-HMWNC) showed in vitro antioxidant activity on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals (IC50 = 512.6±14.4 μg/mL) while others showed less than 50% inhibition at 1000 μg/mL. The lower the molecular weight of chitosan, the higher was the antioxidant activity. The study showed that the encapsulation of COS molecules in HMWNCcould be used as a simple and effective way of enhancing the antioxidant activity of COS.

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Published

2021-03-01

How to Cite

Adhikari, H. S., Garai, A., Marasini, B. P., Adhikari, R., & Yadav, P. N. (2021). Synthesis and Characterization of High Molecular Weight Chitosan, and Antioxidant Activity of Its Chitosan Oligosaccharide Encapsulation. Journal of Nepal Chemical Society, 42(1), 29–38. https://doi.org/10.3126/jncs.v42i1.35326

Issue

Section

Research Article