Biogenic Synthesis of Silver Nanoparticles Using Terminalia chebula Retz. Leaf Extract and Evaluation of Biological Activities

Authors

  • Ramesh Giri Central Department of Chemistry, Tribhuvan University, Kathmandu , Nepal
  • Khaga Raj Sharma Central Department of Chemistry, Tribhuvan University, Kirtipur, Kathmandu, Nepal

DOI:

https://doi.org/10.3126/jncs.v43i1.46957

Keywords:

T. chebula, silver nanoparticles, brine shrimp toxicity, XRD, FTIR, antibacterial, leaf extract, TC-AgNPs

Abstract

Nanoparticles have been used in various fields of science and technology ranging from material science to biotechnology. The formation of nanoparticles has been confirmed through UV-visible spectroscopy (at 420 nm) by the change of color representing surface plasmon resonance. The synthesis of silver nanoparticles by a biogenic method is a novel approach due to its cost-effective, eco-friendly, and large-scale production possibilities. In the present study, silver nanoparticles (TC-AgNPs) were successfully synthesized using Terminalia chebula Retz. (T. chebula) leaf extract. Characterization of green synthesized silver nanoparticles was performed using UV-visible spectroscopy, Fourier transforms infrared (FTIR) spectroscopy, and X-ray diffraction (XRD). The formation of nanoparticles has been confirmed through UV-visible spectroscopy (at 420 nm) by the change of color representing surface plasmon resonance. The crystalline face-centred cubic property of the biosynthesized silver nanoparticles was established using XRD analysis. The XRD data gave the average particle size of 6.1 nm.  The functional groups such as -OH, C=O, =NH were found responsible for reducing silver ions and helping to stabilize nanoparticles which were analyzed using FTIR spectroscopy. As the silver nanoparticles possess diverse applications, TC-AgNPs were investigated for antioxidant, antibacterial, and cytotoxic activity. The results showed TC-AgNPs showed potential antioxidant (IC50=312.8 ± 2.28 µg/mL) and antibacterial activities against four pathogenic bacteria like Staphylococcus aureus, Acinetobacter baumannii, Salmonella typhi, and Escherichia coli. Also, the silver nanoparticles exhibited moderate cytotoxicity (LC50= 477.53 ± 0.684 µg/mL) against brine shrimps nauplii in a dose-dependent manner. 

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Published

2022-08-30

How to Cite

Giri, R., & Sharma, K. (2022). Biogenic Synthesis of Silver Nanoparticles Using Terminalia chebula Retz. Leaf Extract and Evaluation of Biological Activities . Journal of Nepal Chemical Society, 43(1), 54–68. https://doi.org/10.3126/jncs.v43i1.46957

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Section

Research Article