Phytochemical Screening, Antioxidant, Antibacterial, and α-Amylase Inhibitory Activity of Moringa oleifera Lam. Leaves

Authors

  • Surya Kant Kalauni Central Department of Chemistry, Tribhuvan University, Kirtipur, Kathmandu, Nepal
  • Lekh Nath Khanal Prithvi Narayan Campus, Tribhuvan University, Pokhara, Nepal
  • Prakash Thapa Central Department of Chemistry, Tribhuvan University, Kirtipur, Kathmandu, Nepal
  • Keshari Kunwor Central Department of Chemistry, Tribhuvan University, Kirtipur, Kathmandu, Nepal

DOI:

https://doi.org/10.3126/jncs.v43i2.53360

Keywords:

Antimicrobial, Antioxidant, α-Amylase Inhibition, Moringa oleifera

Abstract

Moringa oleifera is a perennial deciduous plant abundant in tropical countries that contains many important bioactive compounds. This study aimed to evaluate phytochemical analysis, antioxidant, antibacterial, and α-amylase inhibitory activities of the methanol and hexane leaf extracts of the plant collected from Nepal. Phytochemical screening of the extracts revealed the presence of a wide spectrum of secondary metabolites such as alkaloids, flavonoids, phenolics, saponins, tannins, terpenoids, etc. Methanol and hexane extracts showed the presence of significant quantities of total phenolics (207.75± 2.75 mg GAE/g, and 137.09 ± 1.1 mg GAE/g) and total flavonoids (94.56 ± 1.88 mg QE/g, and 82.71 ± 1.47 mg QE/g) respectively. The methanol extract exhibited higher 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity. The half-maximal concentration causing 50% inhibition of the radical (IC50) of methanol and hexane extracts was 39.19 ± 0.33 and 61.07 ± 1.46 μg/mL respectively which are comparable to that of standard ascorbic acid (28.90 ± 0.24 μg/mL. Methanol extract of M. oleifera leaves showed moderate antibacterial activity against Staphylococcus aureus (ATCC25923), Bacillus cereus, and Klebsiella pneumonia (ATCC700603). In-vitro antidiabetic activity was performed by a starch-iodine method using α-amylase enzyme and methanol extract showed significant antidiabetic activity (IC50 value 31.78 ± 0.52 μg/mL). The results of this study corroborate the potential application of the plant in traditional medicine and the drug discovery process.

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Published

2023-04-25

How to Cite

Kalauni, S. K., Khanal, L. N., Thapa, P., & Kunwor, K. (2023). Phytochemical Screening, Antioxidant, Antibacterial, and α-Amylase Inhibitory Activity of Moringa oleifera Lam. Leaves. Journal of Nepal Chemical Society, 43(2), 141–150. https://doi.org/10.3126/jncs.v43i2.53360

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Section

Research Article