Bacteriological Profile of Neonatal Sepsis and Antibiogram of the Isolates

  • Roshan Parajuli St. Xaviers College, Kathmandu
  • Narayan Dutt Pant Grande International Hospital, Kathmandu
  • Raju Bhandari Golden Gate International College, Kathmandu
  • Anil Giri Golden Gate International College, Kathmandu
  • Suman Rai Tri-Chandra Multiple Campus, Kathmandu
  • Ganesh Prasad Acharya Paropakar Maternity and Women's Hospital, Kathmandu
  • Pradeep Kumar Shah Tri-Chandra Multiple Campus, Kathmandu
Keywords: Neonatal sepsis, Staphylococcus epidermidis, Escherichia coli, Nepal

Abstract

Introduction: Neonatal sepsis is a serious problem in developing countries like Nepal. The main objectives of this study were to determine the bacteriological profile of neonatal sepsis, to determine the antimicrobial susceptibility patterns of the causative agents and to evaluate the association between the neonatal sepsis and the different characteristics of the neonates.

Methods: A hospital based cross-sectional study was conducted among a total of 450 neonates suspected of suffering from sepsis. Blood culture was performed using statdard microbiological techniques. The colonies grown were identified on the basis of colony morphology, Gram’s stain and biochemical tests. The antimicrobial susceptiblility testing was performed by Kirby Bauer disc diffusion method.

Results: Out of total 450 blood samples, 92 (20.4%) were culture positive. Of which,  16 (17.4%) samples contained  gram negative bacteria and 76 (82.6%) samples contained gram positive cocci. The most common bacterial pathogens isolated were Staphylococcus epidermidis (67.4%) followed by Escherichia coli (13%). All gram positive cocci were susceptible to vancomycin, while all gram negative bacilli were sensitive to amikacin. There was statistically significant relationship between neonatal sepsis and gestation age of neonates.

Conclusions: Neonatal sepsis is still present as a serious problem in Nepal. Staphylococcus epidermidis was the most common cause of the neonatal sepsis. Prematurely delivered neonates should be given more care, as they are more prone to suffering from neonatal sepsis. Vancomycin and amikacin can be used as the drugs of choice for preliminary treatment of neonatal sepsis in our settings.

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Author Biographies

Roshan Parajuli, St. Xaviers College, Kathmandu
Department of Microbiology
Narayan Dutt Pant, Grande International Hospital, Kathmandu
Medical Microbiologist, Department of Microbiology
Raju Bhandari, Golden Gate International College, Kathmandu
Department of Microbiology
Anil Giri, Golden Gate International College, Kathmandu
Department of Microbiology
Suman Rai, Tri-Chandra Multiple Campus, Kathmandu
Department of Microbiology
Pradeep Kumar Shah, Tri-Chandra Multiple Campus, Kathmandu
Department of Microbiology
Published
2017-10-04
How to Cite
Parajuli, R., Pant, N., Bhandari, R., Giri, A., Rai, S., Acharya, G., & Shah, P. (2017). Bacteriological Profile of Neonatal Sepsis and Antibiogram of the Isolates. Journal of Nepal Paediatric Society, 37(1), 5-9. https://doi.org/10.3126/jnps.v37i1.16303
Section
Original Articles