Quantitation of &alpha;<sub>1A</sub> and &alpha;<sub>1D</sub>-adrenoceptor mRNA in prostate tissues from patients with symptomatic benign prostatic hyperplasia

  • B Acharya Laboratory of Cell and Gene Therapy, Institute for Advanced Medical Sciences, Hyogo College of Medicine, Nishinomiya-shi, Hyogo
  • H Okada Department of Urology, Dokkyo Medical University Koshigaya Hospital, Koshigaya
  • G Aryal Department of Pathology, KIST Medical College, Kathmandu
  • T Shirakawa International Center for Medical Research and Treatment, Kobe University School of Medicine, Hyogo
  • N Hinata Division of Urology, Shinko Hospital, Hyogo
  • A Gotoh Laboratory of Cell and Gene Therapy, Institute for Advanced Medical Sciences, Hyogo College of Medicine, Nishinomiya-shi, Hyogo
Keywords: Adrenoceptor subtypes, Benign prostatic hyperplasia, Quantitative reverse transcription-polymerase chain reaction

Abstract

Background: To treat symptomatic benign prostatic hyperplasia α1-adrenoceptor antagonists with little antagonism at α1b-adrenoceptor were used to avoid orthostatic hypotension. In benign prostatic hyperplasia tissues α1D-adrenoceptor are thought to predominate, but in the Japanese experience, either α1A- or α1D- adrenoceptor antagonists can alleviate benign prostatic hyperplasia symptoms. We hypothesized that prostatic expression of α1A- and α1D-adrenoceptor varies quantitatively between patients.

Materials and Methods: We immunohistochemically localized α1A- and α1D-adrenoceptor within benign prostatic hyperplasia tissues, and quantitated mRNA expression for these subtypes by real-time quantitative reverse transcription-polymerase chain reaction.

Results: Immunohistochemistry detected both subtypes in stromal but not detected epithelial cells. Copy numbers of α1A-adrenoceptor mRNA in benign prostatic hyperplasia tissue were significantly higher than those of α1D-adrenoceptor mRNA. Among patients; the ratio of α1A- to α1D-adrenoceptor mRNA ranged from 1.0 to 8.4.

Conclusion: An ideal therapeutic antagonist for treating benign prostatic hyperplasia symptoms should block both α1A- and α1D-adrenoceptor

Keywords: Adrenoceptor subtypes; Benign prostatic hyperplasia; Quantitative reverse transcription-polymerase chain reaction

DOI: 10.3126/jpn.v1i1.4439

Journal of Pathology of Nepal (2011) Vol.1, 1-7

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Acharya, B., Okada, H., Aryal, G., Shirakawa, T., Hinata, N., & Gotoh, A. (1). Quantitation of &alpha;<sub>1A</sub> and &alpha;<sub>1D</sub&gt;-adrenoceptor mRNA in prostate tissues from patients with symptomatic benign prostatic hyperplasia. Journal of Pathology of Nepal, 1(1), 1-7. https://doi.org/10.3126/jpn.v1i1.4439
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