Quantitation of α<sub>1A</sub> and α<sub>1D</sub>-adrenoceptor mRNA in prostate tissues from patients with symptomatic benign prostatic hyperplasia
Background: To treat symptomatic benign prostatic hyperplasia α1-adrenoceptor antagonists with little antagonism at α1b-adrenoceptor were used to avoid orthostatic hypotension. In benign prostatic hyperplasia tissues α1D-adrenoceptor are thought to predominate, but in the Japanese experience, either α1A- or α1D- adrenoceptor antagonists can alleviate benign prostatic hyperplasia symptoms. We hypothesized that prostatic expression of α1A- and α1D-adrenoceptor varies quantitatively between patients.
Materials and Methods: We immunohistochemically localized α1A- and α1D-adrenoceptor within benign prostatic hyperplasia tissues, and quantitated mRNA expression for these subtypes by real-time quantitative reverse transcription-polymerase chain reaction.
Results: Immunohistochemistry detected both subtypes in stromal but not detected epithelial cells. Copy numbers of α1A-adrenoceptor mRNA in benign prostatic hyperplasia tissue were significantly higher than those of α1D-adrenoceptor mRNA. Among patients; the ratio of α1A- to α1D-adrenoceptor mRNA ranged from 1.0 to 8.4.
Conclusion: An ideal therapeutic antagonist for treating benign prostatic hyperplasia symptoms should block both α1A- and α1D-adrenoceptor
Keywords: Adrenoceptor subtypes; Benign prostatic hyperplasia; Quantitative reverse transcription-polymerase chain reaction
Journal of Pathology of Nepal (2011) Vol.1, 1-7