VNTR molecular typing of salmonella enterica serovar typhi isolates in Kathmandu valley

  • B Acharya Laboratory of Cell and Gene Therapy Institute for Advanced Medical Sciences, Hyogo College of Medicine Nishinomiya-shi, Hyogo
  • G Aryal KIST Medical College and Teaching Hospital, Lalitpur
  • N Banjade Sidhhartha Polyclinic, Kathmandu
  • S Sharma Institute of Medicine, Tribhuwan University Teaching Hospital, Kathmandu
  • BM Pokharel Institute of Medicine, Tribhuwan University Teaching Hospital, Kathmandu
  • A Gotoh Laboratory of Cell and Gene Therapy Institute for Advanced Medical Sciences, Hyogo College of Medicine Nishinomiya-shi, Hyogo
Keywords: Salmonella enterica, Variable number Tandem Repeat, Typhoid fever

Abstract

Background: Typhoid fever continues to be a worldwide health problem, especially in developing countries. Effective epidemiological surveillance is needed to monitor the presence and spread of disease.

Materials and Methods: Variable number tandem repeats (VNTR) was performed for Salmonella enterica serovar typhi by multiplex-PCR in 28 Nepalese isolates of sporadic typhoid fever.

Results: From all 28 total isolates, we could identify 12 VNTR profiles among the isolates, signifying multiple variants in circulation within the region.

Conclusion: The VNTR-based typing assay for serovar typhi isolates can be used during an outbreak of enteric fever. The typing could eventually form the basis of an effective epidemiological surveillance system for developing rational strategies to control typhoid fever.

DOI: http://dx.doi.org/10.3126/jpn.v2i3.6026

JPN 2012; 2(3): 220-223

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How to Cite
Acharya, B., Aryal, G., Banjade, N., Sharma, S., Pokharel, B., & Gotoh, A. (1). VNTR molecular typing of salmonella enterica serovar typhi isolates in Kathmandu valley. Journal of Pathology of Nepal, 2(3), 220-223. https://doi.org/10.3126/jpn.v2i3.6026
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Original Articles