In Vitro Regeneration of Phyllanthus amarus Schum. and Thonn.: An Important Medicinal Plant

Authors

  • A. Sen Plant Biotechnology lab. No.5, Department of Botany, University of Rajasthan, Jaipur
  • M.M. Sharma Plant Biotechnology lab. No.5, Department of Botany, University of Rajasthan, Jaipur
  • D. Grover Plant Biotechnology lab. No.5, Department of Botany, University of Rajasthan, Jaipur
  • A. Batra Plant Biotechnology lab. No.5, Department of Botany, University of Rajasthan, Jaipur

DOI:

https://doi.org/10.3126/on.v7i1.2557

Keywords:

In vitro regeneration, multiple shoots, nodal segments, Phyllanthus amarus

Abstract

An efficient in vitro plant regeneration protocol was developed for the medicinally potent plant species Phyllanthus amarus Schum. and Thonn. (Euphorbiaceae) using nodal segment as explant. Maximum multiplication of shoots (15.275±0.96) was achieved on Murashige and Skoog’s medium supplemented with BAP (0.5 mg/l) after 3-4 weeks of inoculation. The shoots were separated from cluster and subcultured for their elongation on the same medium. In vitro flowering was also observed on the elongated shoots after 3–4 weeks of sub culturing on the shoot elongation medium. In vitro rooting was obtained on half strength MS medium supplemented with IBA (0.5 mg/l).  Regenerated plants were successfully hardened and acclimatized, 80 % of plantlets survived well under natural conditions after transplantation.

Key words: In vitro regeneration, multiple shoots, nodal segments, Phyllanthus amarus

DOI: 10.3126/on.v7i1.2557

Our Nature (2009) 7:110-115

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How to Cite

Sen, A., Sharma, M., Grover, D., & Batra, A. (2010). In Vitro Regeneration of Phyllanthus amarus Schum. and Thonn.: An Important Medicinal Plant. Our Nature, 7(1), 110–115. https://doi.org/10.3126/on.v7i1.2557

Issue

Vol. 7 No. 1 (2009)

Section

Articles