The Molecular Mechanisms of Human Osteosarcoma Cell Apoptosis Induced by Arsenic Trioxide and Vincristine

Authors

  • Xiao-Guang Yao Third Hospital, Hebei Medical University, Shijiazhuang, PR China
  • Wei-Hua Zhang Third Hospital, Hebei Medical University, Shijiazhuang, PR China
  • Gui-Song Zhao Third Hospital, Hebei Medical University, Shijiazhuang, PR China
  • Jia-Chen Yao Hebei Medical University, Shijiazhuang, PR China
  • Peng-Huan Wu Third Hospital, Hebei Medical University, Shijiazhuang, PR China
  • Shu-Xia Song Hebei Medical University, Shijiazhuang, PR China
  • Su-Yuan Liu Third Hospital, Hebei Medical University, Shijiazhuang, PR China
  • Sigdel Arun B.P. Koirala Memorial Cancer Hospital, Chitwan, Nepal

DOI:

https://doi.org/10.3126/njc.v1i1.25622

Keywords:

Vincristine, arsenic trioxide, MG-63 cell line, apoptosis, cell cycle, PCNA, cyclin D1, cyclin E, Bcl-2, Bax

Abstract

Background: Arsenic trioxide (As2O3) and vincristine (VCR) have been used for the treatment of tumors. But little is known about the mechanisms and whether they induce apoptosis of human osteosarcoma MG-63 cells.

Methods: MG-63 cells were treated with As2O3 and VCR, respectively. Their effects on cell apoptosis and cell cycle arrest were evaluated by MTT assay and flow cytometry. Their effects on the expressions of apoptosis-related proteins PCNA, Bcl-2, Bax, caspase-3, cyclin D1 and cyclin E were investigated by Western-blot.

Results: Both As2O3 and VCR have time- and dose-dependent effects on apoptosis and cell arrest of MG-63 cells. As2O3 induces MG-63 cell arrest in S phase, whereas VCR induces MG-63 cell arrest in G2/M phase. Both As2O3 and VCR suppress the expression of PCNA, cyclin D1, cyclin E and Bcl-2, and increase the expression of Bax and casepase-3.

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Author Biographies

Xiao-Guang Yao, Third Hospital, Hebei Medical University, Shijiazhuang, PR China

Department of Orthopedic Oncology

Wei-Hua Zhang, Third Hospital, Hebei Medical University, Shijiazhuang, PR China

Department of Orthopedic Oncology

Gui-Song Zhao, Third Hospital, Hebei Medical University, Shijiazhuang, PR China

Department of Orthopedic Oncology

Jia-Chen Yao, Hebei Medical University, Shijiazhuang, PR China

Department of Rehabilitation

Peng-Huan Wu, Third Hospital, Hebei Medical University, Shijiazhuang, PR China

Department of Orthopedic Oncology

Shu-Xia Song, Hebei Medical University, Shijiazhuang, PR China

Department of Molecular Biology and Laboratory Animals

Su-Yuan Liu, Third Hospital, Hebei Medical University, Shijiazhuang, PR China

Department of Orthopedic Oncology

Sigdel Arun, B.P. Koirala Memorial Cancer Hospital, Chitwan, Nepal

Department of Surgical oncology, orthopedic oncology unit

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Published

2017-09-26

How to Cite

Yao, X.-G., Zhang, W.-H., Zhao, G.-S., Yao, J.-C., Wu, P.-H., Song, S.-X., Liu, S.-Y., & Arun, S. (2017). The Molecular Mechanisms of Human Osteosarcoma Cell Apoptosis Induced by Arsenic Trioxide and Vincristine. Nepalese Journal of Cancer, 1(1), 13–20. https://doi.org/10.3126/njc.v1i1.25622

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Section

Original Articles