Beta-Lactamases Production in Multi-drug Resistant Acinetobacter species Isolated from Different Clinical Specimens

  • Mary Neupane National College, Khusibu, Kathmandu, Nepal
  • Sudeep K.C. National College, Khusibu, Kathmandu, Nepal
  • Subash Kumar Thakur Paropakar Maternity and Women’s Hospital, Kathmandu, Nepal
  • Om Prakash Panta National College, Khusibu, Kathmandu, Nepal
  • Dev Raj Joshi Tribhuvan University, Kirtipur, Kathmandu, Nepal
  • Santosh Khanal National College, Khusibu, Kathmandu, Nepal
Keywords: Acinetobacter species, Acb complex, ESBL, MBL, AmpC beta-lactamase

Abstract

Objectives: To determine the prevalence of Acinetobacter spp. from different clinical specimens and detect different types of β-lactamase enzymes.

Methods: Different clinical samples were collected and 125 Acinetobacter spp. were isolated.  Various biochemical tests were carried out to speciate the Acinetobacter spp. The antibiotic susceptibility pattern and β-lactamase enzymes like Extended spectrum β-lactamase (ESBL), Metallo β-lactamase (MBL) and AmpC β-lactamase were determined.

Results: Of the total 125 isolates, the most predominant species was Acinetobacter calcoaceticus-A. baumannii (Acb) complex (80%). Highest rate of isolation of Acinetobacter species were from in-patients (neonates’ blood sample).  Among all, 44.8% isolates were found to be MDR with the majority being resistant to aminoglycosides, carbapenems and fluoroquinolones but not to colistin. ESBL, MBL and AmpC beta-lactamase was detected in 43.2%, 15.2% and 1.6% of the isolates respectively.

Conclusion: Acinetobacter calcoaceticus-A. baumannii complex should be considered for detection in hospitalized patients. The analysis of antibiotic susceptibility pattern and β-lactamases would be helpful to establish network surveillance in order to maintain and control the spread of these resistant strains.

Downloads

Download data is not yet available.
Abstract
229
PDF
196

Author Biographies

Mary Neupane, National College, Khusibu, Kathmandu, Nepal

Department of Microbiology

Sudeep K.C., National College, Khusibu, Kathmandu, Nepal

Department of Microbiology

Subash Kumar Thakur, Paropakar Maternity and Women’s Hospital, Kathmandu, Nepal

Department of Pathology

Om Prakash Panta, National College, Khusibu, Kathmandu, Nepal

Department of Microbiology

Dev Raj Joshi, Tribhuvan University, Kirtipur, Kathmandu, Nepal

Central Department of Microbiology

Santosh Khanal, National College, Khusibu, Kathmandu, Nepal

Department of Microbiology

Published
2019-12-07
How to Cite
Neupane, M., K.C., S., Thakur, S., Panta, O., Joshi, D., & Khanal, S. (2019). Beta-Lactamases Production in Multi-drug Resistant Acinetobacter species Isolated from Different Clinical Specimens. Tribhuvan University Journal of Microbiology, 6, 44-50. https://doi.org/10.3126/tujm.v6i0.26583
Section
Articles