Effective use of Penicillin to Improve Culture Yield for Mycobacterium tuberculosis

  • Sneha Pradhan Tri-Chandra Multiple Campus, Ghantaghar, Kathmandu, Nepal
  • Gokarna Raj Ghimire National Tuberculosis Center, Bhaktapur, Nepal
  • Shova Shrestha Tri-Chandra Multiple Campus, Ghantaghar, Kathmandu, Nepal
Keywords: Culture, Penicillin, Contamination, Pulmonary tuberculosis (PTB), LJ Media

Abstract

Objective: To compare LJ media and LJ media with penicillin for the growth of Mycobacterium tuberculosis and contamination, in pulmonary tuberculosis (PTB) suspected patients.

Methods: A total of 300 PTB suspected cases at National Tuberculosis center (NTC) for analyzed for culture and contamination. Early morning sputum samples were collected in sterile leak-proof falcon tube. Digestion, decontamination and homogenization of sputum were done using NALC-NaOH (Modified Petroff method). The sputum sample was processed on LJ media and penicillin added LJ media and incubated at 37. Cultures were examined after 8 weeks.

Results: All the PTB suspected cases were compared in LJ media and LJ media with penicillin, 29.7% (89) were positive, 21% (63) were contaminated on LJ media whereas 41% (123) were positive, 3.7 % (11) were contaminated on penicillin added LJ media. Also, 25 (8%) were 1+ grading, 14 (4.7%) were 2+ grading, whereas 81 (27%) and 45 (15%) were 3+ grading LJ + Penicillin and LJ media respectively.

Conclusion: Contamination was reduced by 17.3% with the addition of penicillin to LJ media. And isolation of total positive cultures was enhanced by 11.3%.

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Author Biographies

Sneha Pradhan, Tri-Chandra Multiple Campus, Ghantaghar, Kathmandu, Nepal

Department of Microbiology

Gokarna Raj Ghimire, National Tuberculosis Center, Bhaktapur, Nepal

Senior Microbiologist

Shova Shrestha, Tri-Chandra Multiple Campus, Ghantaghar, Kathmandu, Nepal

Associate Professor, Department of Microbiology

Published
2019-12-07
How to Cite
Pradhan, S., Ghimire, G., & Shrestha, S. (2019). Effective use of Penicillin to Improve Culture Yield for Mycobacterium tuberculosis. Tribhuvan University Journal of Microbiology, 6, 103-107. https://doi.org/10.3126/tujm.v6i0.26592
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Articles