Evaluation of Trichoderma spp. against Wirestem Disease of Cauliflower Caused by Rhizoctonia solani

Authors

  • Chandra Bahadur Budha Ministry of Agriculture and Livestock Development, Kathmandu, Nepal
  • Sundar Man Shrestha Agriculture and Forestry University, Chitwan, Nepal
  • Hira Kaji Manandhar Agriculture and Forestry University, Chitwan, Nepal
  • Suraj Baidya Nepal Agricultural Research Council, Lalitpur, Nepal

DOI:

https://doi.org/10.3126/aej.v24i01.58179

Keywords:

Biocontrol, cauliflower, rhizoctonia, trichoderma, wirestem disease

Abstract

The biological effectiveness of twenty-eight native isolates of Trichoderma along with two known species (Trichoderma viride and Trichoderma harzianum) were tested against Rhizoctonia solani using dual culture technique. Of them, seven isolates and the two species of Trichoderma were tested in pot culture under net-house conditions against wirestem disease of cauliflower caused by R. solani. Inhibitory effects on dual culture were statistically very significant for all tested Trichoderma. Thirteen Trichoderma isolates exhibited more than 90% bio-control index and twenty-three isolates had more or at par with T. viride and T. harzianum. In an in-vitro trial, isolate from Dhankuta (Pakhribas) was most effective against R. solani as evident by the highest bio-control index at 6 days after incubation. Similar to this, in an in-vivo experiment, tested isolates of Trichoderma contributed more or less in the plant canopy area, root length and shoot circumference attributes. Trichoderma isolates significantly contributed to root dry matter and biomass while there was a highly significant difference in reduction of disease severity. T. harzianum and the isolate from Dhankuta (Pakhribas) were most effective for the control of wirestem disease

Downloads

Download data is not yet available.
Abstract
153
pdf
191

Downloads

Published

2023-06-30

How to Cite

Budha, C. B., Shrestha, S. M., Manandhar, H. K., & Baidya, S. (2023). Evaluation of Trichoderma spp. against Wirestem Disease of Cauliflower Caused by Rhizoctonia solani. Journal of Agriculture and Environment, 24(01), 127–136. https://doi.org/10.3126/aej.v24i01.58179