Marker Assisted Selection for Bacterial Leaf Blight Resistance in Segregating Populations of Cottondora Sannalu

  • Manu Maya Magar Nepal Agricultural Research Institute
  • Ch.V. Durga Rani College of Agriculture, Acharya N. G. Ranga Agricultural University, Rajendranagar, Hyderabad
  • G Auradha College of Agriculture, Acharya N. G. Ranga Agricultural University, Rajendranagar, Hyderabad
Keywords: Rice, F2 population, Bacterial Leaf Blight, Marker Assisted Selection

Abstract

The present investigation was undertaken with the objective to develop high yielding, fine grain rice varieties possessing broad spectrum durable resistance by transferring bacterial leaf blight (BLB) resistant genes viz., xa13 and Xa21 from B95-1. A popular high yielding and fine grain rice variety, MTU1010 (Cottondora Sannalu), susceptible to BLB and B95-1 carrying resistant genes for BLB (xa13 & Xa21 genes) was selected as the parent for crossing. B95-1 was verified for the presence of target genes by using gene linked primers viz., xa13 promotor and pTA 248. These primers were also used to study polymorphism between resistant (B95-1) and susceptible (MTU1010) parents. The cross viz., MTU1010 x B95-1 was affected during Rabi, 2010 and F1 progenies were confirmed during Kharif, 2011. The F1 plants confirmed as true hybrids for both the genes were advanced to F2 generation and foreground selection was done using gene linked markers. Genetic analysis in F2 populations confirmed that the genes (xa13 & Xa21) governing BLB resistance followed Mendelian inheritance. The phenotypic data analysis revealed that the plants carrying two resistance gene combinations (xa13xa13 Xa21Xa21, xa13xa13Xa21xa21) showed BLB resistance (0-2 scale), while the gene combinations viz., Xa13Xa13Xa21Xa21, Xa13Xa13Xa21xa21, and Xa13xa13Xa21Xa21, Xa13xa13Xa21xa21 showed BLB resistance (0.5-3.0).

DOI: http://dx.doi.org/10.3126/ijasbt.v2i3.10570

Int J Appl Sci Biotechnol, Vol. 2(3): 229-237  

 

Downloads

Download data is not yet available.
Abstract
523
PDF
320

Author Biographies

Manu Maya Magar, Nepal Agricultural Research Institute

Biotechnology Division

Rank-Scientist (S1)

Ch.V. Durga Rani, College of Agriculture, Acharya N. G. Ranga Agricultural University, Rajendranagar, Hyderabad

Institute of Biotechnology

Rank- Professor

G Auradha, College of Agriculture, Acharya N. G. Ranga Agricultural University, Rajendranagar, Hyderabad

Institute of Biotechnology

Rank- Principal Scientist

Published
2014-09-25
How to Cite
Magar, M., Rani, C., & Auradha, G. (2014). Marker Assisted Selection for Bacterial Leaf Blight Resistance in Segregating Populations of Cottondora Sannalu. International Journal of Applied Sciences and Biotechnology, 2(3), 229-237. https://doi.org/10.3126/ijasbt.v2i3.10570
Section
Research Articles