In vitro seed germination and Growth of Three Varieties of Black Gram after Ultraviolet-B Radiation

  • K Rajendiran Department of Botany, K.M. Centre for Post Graduate Studies, Pondicherry - 605 008 http://orcid.org/0000-0002-5127-3207
  • K Thiruvarasan Department of Botany, K.M. Centre for Post Graduate Studies, Pondicherry - 605 008
  • R Vijayalakshmi Department of Botany, K.M. Centre for Post Graduate Studies, Pondicherry - 605 008
Keywords: Black gram, In vitro seed germination, varieties, Ultraviolet-B,

Abstract

In vitro seed germination and growth of seedlings was tried with three varieties of black gram (Vigna mungo (L.) Hepper) viz. Vamban-3, Nirmal-7 and T-9 after ultraviolet-B irradiation (UV-B = 2 hours once with 1 hour recovery time @ 12.2 kJ m-2 d-1; ambient = 10 kJ m-2 d-1). Unstressed and UV-B stressed VAMBAN-3 and T-9 seeds both in dry and wet conditions responded to in vitro germination. Unstressed NIRMAL-7 failed to germinate under in vitro culture. UV-B stressed NIRMAL-7 responded to in vitro culture. UV-B irradiation enhanced seedling height at both dry and wet conditions in VAMBAN-3 followed by NIRMAL-7 compared with controls. Root and shoot length of UV-B stressed VAMBAN-3 and NIRMAL-7 performed five to six times better than control. Root and shoot length of T-9 was reduced (2.61 to 8.69 %) below control after UV-B exposure. UV-B stressed VAMBAN-3 under dry and wet exposure accumulated three to six times more plant biomass over controls. UV-B stressed NIRMAL-7 and T-9 dry seeds accumulated less plant biomass by 70.86 % and 12.39 % respectively than their controls. NIRMAL-7 and T-9 under dry UV-B exposure produced two times more leaves than control.

Int J Appl Sci Biotechnol, Vol 4(1): 117-129

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Abstract
419
PDF
334
Published
2016-03-31
How to Cite
Rajendiran, K., Thiruvarasan, K., & Vijayalakshmi, R. (2016). In vitro seed germination and Growth of Three Varieties of Black Gram after Ultraviolet-B Radiation. International Journal of Applied Sciences and Biotechnology, 4(1), 117-129. https://doi.org/10.3126/ijasbt.v4i1.14718
Section
Research Articles