Citric Acid Production by Wild and UV – Treated Strains of Aspergillus niger on Two Different Mineral Salt Media

  • Valentine Enyinna Anyanwu Federal University of Technology, Owerri, Imo State.
  • Phillip O. Okerentugba University of Port Harcourt, P.M.B. 5323, Port Harcourt, Rivers State
Keywords: Citric acid production, Aspergillus niger, sucrose, improved UV-1 strain, kinetic parameters.

Abstract

Microbial production of citric acid by a novel Aspergillus niger EE-12 and its UV – treated strain (UV-1) were carried out in shake flask cultures using mineral salt media containing sucrose or fructose as the carbon and energy sources. The highest citric acid concentration (36.1g/l) was obtained with the UV – treated strain UV-1 after 144 hours in medium containing sucrose and this was significantly higher (p<0.05) than the concentration produced by its parent strain EE-12. Citric acid production using medium containing sucrose (Sucrose salt medium) was significantly higher (p<0.05) than from medium containing fructose (Fructose salt medium) for both organisms. Product and growth yield coefficients (YCA/S, YCA/F, YCA/X, YX/S, YX/F) and volumetric rates (QF, QS, QCA, QX) were studied and compared for each of the test organisms based on the media (sucrose and fructose salt media) using students t-test analysis. This research indicated that the fungi strains are efficient for citric acid production using sucrose based medium.

DOI: http://dx.doi.org/10.3126/ijasbt.v1i3.8358

Int J Appl Sci Biotechnol, Vol. 1(3) 2013 : 145-153

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Author Biographies

Valentine Enyinna Anyanwu, Federal University of Technology, Owerri, Imo State.

Department of Microbiology.

Assistant Lecturer

Phillip O. Okerentugba, University of Port Harcourt, P.M.B. 5323, Port Harcourt, Rivers State
Department of Microbiology.
Published
2013-09-25
How to Cite
Anyanwu, V., & Okerentugba, P. (2013). Citric Acid Production by Wild and UV – Treated Strains of Aspergillus niger on Two Different Mineral Salt Media. International Journal of Applied Sciences and Biotechnology, 1(3), 145-153. https://doi.org/10.3126/ijasbt.v1i3.8358
Section
Research Articles