An Investigation on principle biochemical components, photosynthetic pigments, nucleic acid and enzymatic activities of axenic culture of Scytonema sp. treated with two PAHs: Acenapthene and Fluoranthene

  • Miral Patel Sophisticated Instrumentation Center for Applied Research and Testing (SICART), Vallabh Vidya Nagar, Gujarat India- 388 120.
  • Nirmal Kumar J.I P.G. Department of environmental Science and Technology, Institute of Science and Technology for Advanced Studies and Research (ISTAR), Vallabh Vidya Nagar, Gujarat India- 388 120.
  • K. K. Tiwari Sophisticated Instrumentation Center for Applied Research and Testing (SICART), Vallabh Vidya Nagar, Gujarat India- 388 120.
Keywords: PAHs, Scytonema sp., biochemical, enzyme activities, pigments, DNA, RNA, C/N ratio

Abstract

The fresh water cyanobacteria Scytonema sp. was cultivated in a laboratory scale in the presence of various concentrations (2.5, 5, 10, 20 ppm) of two polycyclic hydrocarbons in order to assess the influence of the pollutant on the growth and certain physiological responses of the cyanobacteria. The algal cells were analyzed for chlorophyll a, carotenoids, phycobilliproteins, proteins, amino acid, nucliec acid, C/N ratio and elemental composition, carbohydrate and different enzymes at four days interval up to 16 days. According to our results, Scytonema sp. was significantly affected by the pollution with regard to the different physiological parameters examined, and this significance may be negative, positive or variable. The effect of the pollutant on carbohydrate, and the total amount of amino acids, proteins was negative, however, the composition of the phenol increase with raise in PAHs concentration. A positive effect of the pollutant on cellular C/N ratio was observed up to certain doses of PAHs.

DOI: http://dx.doi.org/10.3126/ijasbt.v2i1.9784

Int J Appl Sci Biotechnol, Vol. 2(1): 34-40

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Abstract
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Published
2014-03-25
How to Cite
Patel, M., J.I, N., & Tiwari, K. (2014). An Investigation on principle biochemical components, photosynthetic pigments, nucleic acid and enzymatic activities of axenic culture of Scytonema sp. treated with two PAHs: Acenapthene and Fluoranthene. International Journal of Applied Sciences and Biotechnology, 2(1), 34-40. https://doi.org/10.3126/ijasbt.v2i1.9784
Section
Research Articles