Cellular Alteration of the Oral Mucosa due to Orthodontic Appliances and Tobacco Smoking: A Cytomorphometric Study

  • Vinay Marla Penang International Dental College, Penang, Malaysia http://orcid.org/0000-0001-6673-9236
  • Dharanidhar Baral BP Koirala Institute of Health Sciences, Dharan, Nepal
  • Prabhat Ranjan Pokharel BP Koirala Institute of Health Sciences, Dharan, Nepal
  • Ashish Shrestha BP Koirala Institute of Health Sciences, Dharan, Nepal
  • Rajesh Gyawali BP Koirala Institute of Health Sciences, Dharan, Nepal
  • Jamal Giri BP Koirala Institute of Health Sciences, Dharan, Nepal
Keywords: cytomorphometry; fixed orthodontic treatment; tobacco smoking.

Abstract

Introduction: Fixed orthodontic treatment causes chronic low grade irritation on the oral mucosa. Tobacco smoking is considered to be a risk factor in the development of oral cancer. This study involves a cytomorphometric analysis on the buccal mucosal cells in patients undergoing fixed orthodontic treatment who are tobacco smokers.

Methods: Exfoliated buccal mucosal cells were obtained from orthodontic patients (smokers & non-smokers) as well as smokers and non-smokers who are not undergoing any orthodontic treatment. Smears were stained with rapid PAP stain and digital images of 50 cells were analysed using the Image J software.

Results: Statistical analysis revealed that there were significant increase in the values of nuclear and cell diameter between non-smokers undergoing orthodontic treatment and smokers (p<0.001 & p=0.014 respectively), and between non-smokers undergoing orthodontic treatment and smokers undergoing orthodontic treatment (p<0.001 & p=0.015 respectively). Also, nuclear area and cell area showed significant differences between many of the study groups.

Conclusion: It can be concluded that there are nuclear and cellular alterations in patients undergoing fixed orthodontic treatment who are smokers

Downloads

Download data is not yet available.
Abstract
78
PDF
37

Author Biographies

Vinay Marla, Penang International Dental College, Penang, Malaysia

Department of Oral Pathology

Dharanidhar Baral, BP Koirala Institute of Health Sciences, Dharan, Nepal

Department of Community Medicine

Prabhat Ranjan Pokharel, BP Koirala Institute of Health Sciences, Dharan, Nepal

Department of Orthodontics

Ashish Shrestha, BP Koirala Institute of Health Sciences, Dharan, Nepal

Department of Oral Pathology

Rajesh Gyawali, BP Koirala Institute of Health Sciences, Dharan, Nepal

Department Orthodontics

Jamal Giri, BP Koirala Institute of Health Sciences, Dharan, Nepal

Department of Orthodontics

Published
2019-10-01
How to Cite
Marla, V., Baral, D., Pokharel, P., Shrestha, A., Gyawali, R., & Giri, J. (2019). Cellular Alteration of the Oral Mucosa due to Orthodontic Appliances and Tobacco Smoking: A Cytomorphometric Study. Journal of College of Medical Sciences-Nepal, 15(3), 206-210. https://doi.org/10.3126/jcmsn.v15i3.22620
Section
Original Articles