Isolation of Plasma Proteins from Bovine Blood by Cold Ethanol Precipitation and Anion Exchange Chromatography

Authors

  • Patricia Adamma Ekwumemgbo Department of Chemistry
  • James Adagadzu Kagbu Department of Chemistry
  • Andrew Jonathan Nok Department of Biochemistry
  • Israel Kehinde Omoniyi School of Remedical Studies
  • Paul Ocheme Ameh Department of Chemistry
  • Nathaniel Oladunni Department of Chemistry

DOI:

https://doi.org/10.3126/jncs.v26i0.3624

Keywords:

Plasma, Chromatography, Electrophoresis, Albumin, Fibrinogen, Globulin

Abstract

Plasma (100.00 ml) obtained from bovine blood by centrifugation was fractionated
into seven precipitates by cold ethanol precipitation. The yield (amount) of proteins in the
precipitates calculated from the standard curve of BSA was 1160.83 mg, 806.57 mg,
1149.94 mg, 8.79 mg, 19.88 mg, 21.98 mg, and 13.97 mg respectively, while the total
amount of protein obtained was 3180.00 mg. The precipitates were fractionated into 25
fractions each by Anion Exchange Chromatography (AEC) and the amount of protein in
each fraction was obtained by Bradford protein assay. SDS-PAGE analysis was performed
on the fractions with proteins and their estimated molecular weights were obtained with the
aid of the molecular weight marker. The result indicated that cold ethanol precipitation and
anion exchange chromatographic techniques are valuable tools for the purification of
bovine blood to obtain high grade α, β and γ-fibrinogen, IgM (μ-globulin), IgG (γ -globulin),
alpha (α –globulin), β-globulin (E-globulin) and albumin.

Keywords: Plasma; Chromatography; Electrophoresis; Albumin; Fibrinogen; Globulin

DOI: 10.3126/jncs.v26i0.3624

Journal of Nepal Chemical Society
Vol. 26, 2010
Page: 2-12

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Author Biographies

Patricia Adamma Ekwumemgbo, Department of Chemistry

professor, Department of Chemistry, Ahmadu University,  Zaria, Nigeria

James Adagadzu Kagbu, Department of Chemistry

professor, Department of Chemistry, Ahmadu University,  Zaria, Nigeria

Andrew Jonathan Nok, Department of Biochemistry

professor, Department of Biochemistry, Ahmadu Bello University, Zaria

Israel Kehinde Omoniyi, School of Remedical Studies

Professor, Ahmadu Bello University, School of Remedial Studies Funtua

Paul Ocheme Ameh, Department of Chemistry

Professor, Department of Chemistry, Ahmadu University,  Zaria, Nigeria

Nathaniel Oladunni, Department of Chemistry

Professor, Department of Chemistry, Ahmadu University,  Zaria, Nigeria

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How to Cite

Ekwumemgbo, P. A., Kagbu, J. A., Nok, A. J., Omoniyi, I. K., Ameh, P. O., & Oladunni, N. (2010). Isolation of Plasma Proteins from Bovine Blood by Cold Ethanol Precipitation and Anion Exchange Chromatography. Journal of Nepal Chemical Society, 26, 2–12. https://doi.org/10.3126/jncs.v26i0.3624

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