Improvised Media for In Vitro Pollen Germination of some Species of Apocynaceae

  • Reshmi Chatterjee Birbal Sahni Institute of Palaeobotany, 53 University Raod, Lucknow – 226 007, U.P
  • Satadip Sarkar Department of Botany, Bethune College, Kolkata- 700006, West Bengal
  • GM Narasimha Rao 3Department of Botany, Andhra University, Visakhapatnam-530 003, Andhra Pradesh
Keywords: In vitro, Pollen Germination, Apocynaceae, Sitting-Drop Culture, Basic Culture


Pollen germination forms one of the most important stage post pollination prior to fertilization. This is essential for proper seed setting and seed development. In vitro pollen germination test is the most reliable way of assessing the pollen viability. In the present study pollen grains of seven genera under Apocynaceae family namely, Allamanda, Alstonia, Catharanthus, Nerium, Plumeria, Thevetia and Tabernaemontana were tested in some basic cultural media, such as Brewbaker’s media, 6% Glucose solution, 4% Calcium Nitrate solution and 3% Boron solution. Alstonia pollen grains exhibited highest percentage of germination rate in all the cultural media. Glucose and Brewbaker’s media is found to be highly suitable for efficient pollen germination in all the genera. Boron solution is effective for germination of pollen grains of tree species. In vitro pollen germination can be easily carried out in laboratories. These results can be utilised in plant breeding programmes to improve cultivar and varieties.


International Journal of Environment Vol.3(3) 2014: 146-153


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How to Cite
Chatterjee, R., Sarkar, S., & Rao, G. (2014). Improvised Media for In Vitro Pollen Germination of some Species of Apocynaceae. International Journal of Environment, 3(3), 146-153.
Research Papers