Heavy Metal Tolerance of Bacterial Isolates from Solid Waste Dumping Sites in Abuja, Nigeria

Authors

  • Beatrice O. Ojiego Department of Environmental Biotechnology and Bio-conservation, National Biotechnology Development Agency, Abuja, Nigeria
  • Josephine Madu Department of Environmental Biotechnology and Bio-conservation, National Biotechnology Development Agency, Abuja, Nigeria
  • Obianuju P. Ilo Department of Environmental Biotechnology and Bio-conservation, National Biotechnology Development Agency, Abuja, Nigeria
  • Joshua A. Odoh Department of Microbiology, Federal University Lokoja, Kogi State, Nigeria
  • Ephraim K. Audu Department of Biology, Ahmadu Bello University, Zaria, Kaduna State, Nigeria
  • Twan Ishaku Department of Biology, Ahmadu Bello University, Zaria, Kaduna State, Nigeria
  • Shauibu A. Abdullahi Department of Biology, Ahmadu Bello University, Zaria, Kaduna State, Nigeria
  • Ibrahim M.K. Gadzama Department of Biology, Ahmadu Bello University, Zaria, Kaduna State, Nigeria
  • Paul Bolorunduro Department of Biology, Ahmadu Bello University, Zaria, Kaduna State, Nigeria
  • Elijah Ella Department of Microbiology, Ahmadu Bello University, Zaria, Kaduna State, Nigeria
  • Gideon I. Ogu Department of Microbiology, Federal University Lokoja, Kogi State, Nigeria https://orcid.org/0000-0002-4594-8386

DOI:

https://doi.org/10.3126/ije.v12i1.52441

Keywords:

Bacteria, Heavy metals, Metal resistance, Solid waste dumping sites

Abstract

Bacteria have special bio-mechanism to resist toxic heavy metals. This study investigated heavy metal tolerance potentials of bacterial isolates from solid waste dumping sites (Abaji, Bwari, Gosa, Gwagwalada, Kuje, and Kwali) in Abuja, Nigeria. Soil samples were randomly collected from each location using soil augers at depths of 0 – 15, 15 – 35 and 35 – 45 cm. They were analyzed bacteriologically using cultural/biochemical techniques and chemically by exposing the isolates to graded concentrations (50 - 400 μg/mL) of chromium (Cr), nickel (Ni) and lead (Pb) on nutrient agar for heavy metal tolerance test. Statistical analysis revealed a significant decrease (p<0.05) in the heterotrophic bacterial count with soil depth; with the highest counts (6.89 × 109 CFU/g) noted at 0 – 15 cm (Gosa) and lowest (1.32 × 103 CFU/g) found at 30 – 45 cm (Kuje). The isolated bacteria (n=54) were Proteus (33.3 %), Providencia (29.6 %), Pseudomonas (16.6 %), Bacillus (9.3 %), Micrococcus (5.5 %), Escherichia coli (2.1 %), Enterobacter (2.1 %), and Serratia (2.1 %). All these isolates except Micrococcus spp., Enterobacter spp., Escherichia coli and Serratia spp. displayed 100 % resistance to Cr, Ni and Pb at ≥ 200 μg/mL with MICs (μg/mL) being 850 – 1700 (Pseudomonas Proteus spp.), 950 – 2250 (Pseudomonas Bacillus spp.) and 900 – 1750 (Pseudomonas Bacillus spp.), respectively. Majority of these bacteria (24.1 - 38.9 %) were from Gosa and Gwagwalada dumping sites. Our findings suggested these bacteria could be promising for remediation of the heavy metals in the sites.

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Published

2023-02-27

How to Cite

Ojiego, B. O., Madu, J., Ilo, O. P., Odoh, J. A., Audu, E. K., Ishaku, T., Abdullahi, S. A., Gadzama, I. M., Bolorunduro, P., Ella, E., & Ogu, G. I. (2023). Heavy Metal Tolerance of Bacterial Isolates from Solid Waste Dumping Sites in Abuja, Nigeria. International Journal of Environment, 12(1), 30–46. https://doi.org/10.3126/ije.v12i1.52441

Issue

Section

Research Article