Bioassay Guided Isolation of Free Radical Scavenging Agent from the Bark of Bridelia retusa

Authors

  • Khaga Raj Sharma Central Department of Chemistry, Tribhuvan University, Kirtipur and Birendra Multiple Campus, TU Bharatpur, Chitwan
  • Achyut Adhikari H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences (ICCBS), University of Karachi
  • M. Iqbal Choudhary H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences (ICCBS), University of Karachi
  • Suresh Awale University of Toyama, Frontier Research Core for Life Sciences, 2630-Sugitani, Tokyo
  • Surya Kant Kalauni Central Department of Chemistry, Tribhuvan University, Kirtipur

DOI:

https://doi.org/10.3126/jist.v20i1.13916

Keywords:

Bridelia retusa, tambulin, free radical scavenging, DPPH

Abstract

Bioassay guided fractionation of ethyl acetate soluble fraction of methanolic extract of Bridelia retusa yielded tambulin (1), beta-sitosterol (2), and beta-sitosterol glucoside (3), and their structures were elucidated using modern spectroscopic techniques. Compounds 1-3 were evaluated for their antioxidant activity and compound 1 was found to be potent antioxidant. The DPPH assay showed that the compound 1 has potent antioxidant activity with IC50 166.15±1.92 SEM [muM] and the radical scavenging activity was found to be 86.03% where as the standard butylated hydroxy toluene (BHT) has IC50 value 128.83±2.1 SEM [muM]. The radical scavenging activity of standard butylated hydroxy toluene was about 86% .The total phenolic content in ethyl acetate soluble fraction was found to be 147.20±1.5 mg GAE/gm and the total flavonoid content was found to be 16.64±0.00 mg QE/gm.

Journal of Institute of Science and Technology, 2015, 20(1): 97-101

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Published

2015-11-25

How to Cite

Sharma, K. R., Adhikari, A., Choudhary, M. I., Awale, S., & Kalauni, S. K. (2015). Bioassay Guided Isolation of Free Radical Scavenging Agent from the Bark of Bridelia retusa. Journal of Institute of Science and Technology, 20(1), 97–101. https://doi.org/10.3126/jist.v20i1.13916

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Section

Research Articles