Bioassay Guided Isolation of Free Radical Scavenging Agent from the Bark of Bridelia retusa

Authors

  • Khaga Raj Sharma Central Department of Chemistry, Tribhuvan University, Kirtipur and Birendra Multiple Campus, TU Bharatpur, Chitwan
  • Achyut Adhikari H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences (ICCBS), University of Karachi
  • M. Iqbal Choudhary H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences (ICCBS), University of Karachi
  • Suresh Awale University of Toyama, Frontier Research Core for Life Sciences, 2630-Sugitani, Tokyo
  • Surya Kant Kalauni Central Department of Chemistry, Tribhuvan University, Kirtipur

Keywords:

Bridelia retusa, tambulin, free radical scavenging, DPPH

Abstract

Bioassay guided fractionation of ethyl acetate soluble fraction of methanolic extract of Bridelia retusa yielded tambulin (1), beta-sitosterol (2), and beta-sitosterol glucoside (3), and their structures were elucidated using modern spectroscopic techniques. Compounds 1-3 were evaluated for their antioxidant activity and compound 1 was found to be potent antioxidant. The DPPH assay showed that the compound 1 has potent antioxidant activity with IC50 166.15±1.92 SEM [muM] and the radical scavenging activity was found to be 86.03% where as the standard butylated hydroxy toluene (BHT) has IC50 value 128.83±2.1 SEM [muM]. The radical scavenging activity of standard butylated hydroxy toluene was about 86% .The total phenolic content in ethyl acetate soluble fraction was found to be 147.20±1.5 mg GAE/gm and the total flavonoid content was found to be 16.64±0.00 mg QE/gm.

Journal of Institute of Science and Technology, 2015, 20(1): 97-101

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Published

2015-11-25

How to Cite

Bioassay Guided Isolation of Free Radical Scavenging Agent from the Bark of Bridelia retusa. (2015). Journal of Institute of Science and Technology, 20(1), 97-101. https://doi.org/10.3126/jist.v20i1.13916

Issue

Vol. 20 No. 1 (2015)

Section

Research Articles

License

The copyright of the articles is held by the Institute of Science and Technology, Tribhuvan University (IoST- TU). The views and interpretations in this journal are those of the author(s). They are not attributable to the IoST-TU and do not imply the expression of any opinion concerning the legal status of any country, territory, city, area of its authorities, or concerning the delimitation of its frontiers or boundaries. The corresponding author is responsible for any conflict of interest between authors and others.

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How to Cite

Bioassay Guided Isolation of Free Radical Scavenging Agent from the Bark of Bridelia retusa. (2015). Journal of Institute of Science and Technology, 20(1), 97-101. https://doi.org/10.3126/jist.v20i1.13916