Isolation and Characterization of Soil Myxobacteria from Nepal

  • Nabin Rana Department of Biotechnology, National College, Tribhuvan University, Naya Bazar, Kathmandu
  • Saraswoti Khadka Department of Biotechnology, National College, Tribhuvan University, Naya Bazar, Kathmandu
  • Bishnu Prasad Marasini Department of Biotechnology, National College, Tribhuvan University, Naya Bazar, Kathmandu
  • Bishnu Joshi Department of Biotechnology, National College, Tribhuvan University, Naya Bazar, Kathmandu
  • Pramod Poudel Department of Biotechnology, National College, Tribhuvan University, Naya Bazar, Kathmandu
  • Pramod Poudel Research Division, University Grant Commission (UGC-Nepal), Sanothimi, Bhaktapur
  • Santosh Khanal Department of Biotechnology, National College, Tribhuvan University, Naya Bazar, Kathmandu
  • Niranjan Parajuli Central Department of Chemistry, Tribhuvan University, Kirtipur, Kathmandu,
Keywords: Antimicrobial, Cystobacterineae, Myxobacteria, Soil habitat of Nepal

Abstract

 Realizing myxobacteria as a potential source of antimicrobial metabolites, we pursued research to isolate myxobacteria showing antimicrobial properties. We have successfully isolated three strains (NR-1, NR-2, NR-3) using the Escherichia coli baiting technique. These isolates showed typical myxobacterial growth characteristics. Phylogenetic analysis showed that all the strains (NR-1, NR-2, NR-3) belong to the family Archangiaceae, suborder Cystobacterineae, and order Myxococcales. Furthermore, 16S rRNA gene sequence similarity searched through BLAST revealed that strain NR-1 showed the closest similarity (91.8 %) to the type strain Vitiosangium cumulatum (NR-156939), NR-2 showed (98.8 %) to the type of Cystobacter badius (NR-043940), and NR-3 showed the closest similarity (83.5 %) to the type of strain Cystobacter fuscus (KP-306730). All isolates showed better growth in 0.5-1 % NaCl and pH around 7.0, whereas no growth was observed at pH 9.0 and below 5.0. All strains showed better growth at 32° C and hydrolyzed starch, whereas casein was efficiently hydrolyzed by NR-1 and NR-2. Besides, preliminary antimicrobial tests from crude extracts showed activities against Gram-positive, Gram-negative bacteria, and fungi. Our findings suggest that the arcane soil habitats of Nepal harbor myxobacteria with the capability to produce diverse antimicrobial activities that may be explored to overcome the rapidly rising global concern about antibiotic resistance.

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Published
2019-12-31
How to Cite
Rana, N., Khadka, S., Marasini, B., Joshi, B., Poudel, P., Poudel, P., Khanal, S., & Parajuli, N. (2019). Isolation and Characterization of Soil Myxobacteria from Nepal. Journal of Institute of Science and Technology, 24(2), 7-16. https://doi.org/10.3126/jist.v24i2.27246
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Research Article