Marker Assisted Screening of Nepalese rice for bacterial leaf blight (BLB) resistance

Authors

  • Resham Babu Amgai Biotechnology Division, Nepal Agriculture Research Council, Khumaltar, Lalitpur
  • Raj Kumar Niroula Biotechnology Division, Nepal Agriculture Research Council, Khumaltar, Lalitpur
  • Sumitra Pantha Agriculture Botany Division, Nepal Agriculture Research Council, Khumaltar, Lalitpur
  • Shreya Singh Hamal Biotechnology Division, Nepal Agriculture Research Council, Khumaltar, Lalitpur
  • Bishal Gole Tamang Institute of Agriculture and Animal Science, Tribhuwan University, Rampur, Chitwan
  • Bindeshwar Prasad Sah Biotechnology Division, Nepal Agriculture Research Council, Khumaltar, Lalitpur
  • Madan Raj Bhatta National Plant Genetic Resource Centre, Nepal Agriculture Research Council, Khumaltar, Lalitpur

DOI:

https://doi.org/10.3126/njb.v3i1.14227

Keywords:

Bacterial Leaf Blight, Simple Sequence Repeats, resistant gene, Nepalese rice, Marker Assisted Screening

Abstract

Bacterial Leaf Blight (BLB) is the most important yield limiting factor in Nepalese rice. BLB resistance rice varieties are highly demanding in the country. Breeding efforts for developing disease resistant depends on availability and use of resistant gene donors. Nepalese rice landraces could be the source of resistant gene. Therefore, ninety six Nepalese rice accessions were screened using eight Simple Sequence Repeats (SSR) markers and one Sequence Tagged Sites (STS) marker for presence and absence of BLB resistance gene. We have detected BLB resistance gene Xa-10 on five accessions, Xa-13 on six accessions, Xa-7 on 23 accessions, Xa-3 and Xa-4 on 52 accessions, Xa-5 on 25 accessions, Xa-8 on 30 rice accessions. No any rice accessions tested have Xa-21. Similarly, 17 rice accessions showed three and more than three BLB resistance genes. Presence of Xa-13 on susceptible check variety CNTRL-85033 confirmed that this resistant gene is not working in Nepalese rice field. Therefore, Nepal need to pyramide the BLB resistant genes for durable resistance.

Nepal Journal of Biotechnology. Dec. 2015 Vol. 3, No. 1: 35-39

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Published

2015-12-30

How to Cite

Amgai, R. B., Niroula, R. K., Pantha, S., Hamal, S. S., Tamang, B. G., Sah, B. P., & Bhatta, M. R. (2015). Marker Assisted Screening of Nepalese rice for bacterial leaf blight (BLB) resistance. Nepal Journal of Biotechnology, 3(1), 35–39. https://doi.org/10.3126/njb.v3i1.14227

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Section

Original Research Articles