Detection of Pyuria by Microscopic Urinalysis as a Marker of Pediatric Urinary Tract Infection

  • Dhiraj Shrestha Department of Microbiology, Shi-Gan International College of Science and Technology, Kathmandu, Nepal
  • Pratigya Thapa Department of Microbiology, Kathmandu UpatyakaKhanepani Limited (KUKL), Kathmandu, Nepal
  • Dinesh Bhandari School of Public Health, University of Adelaide, Australia
  • Balkrishna Bhattachan Biotechnology Society Nepal, Bhaktapur, Nepal
  • Hiramani Parajuli Department of Microbiology, Tri-Chandra Multiple College, Kathmandu, Nepal
  • Prakash Chaudary Department of Microbiology, Tri-Chandra Multiple College, Kathmandu, Nepal
  • Vijay Kumar Sharma Department of Biochemistry, Institute of Medicine, TUTH, Kathmandu, Nepal
  • Pradeep Kumar Shah Department of Microbiology, Tri-Chandra Multiple College, Kathmandu, Nepal
Keywords: febrile, marker, microscopic urinalysis, pediatric, pyuria, UTI

Abstract

Globally, different diagnostic tests of urinary tract infection (UTI) are in clinical practices. A reliable test can increase the efficiency of the healthcare system, especially in a developing country like Nepal, reducing cost and time. Thus, we accessed the possibility of pyuria detected by microscopic urinalysis as a marker of pediatric UTI. The prospective study was conducted fromJuly2014 to January 2015 at Alka hospital, Lalitpur. Microscopic urinalysis of 353clean-catch urine samples was done by the wet mount method, followed by urine culture by a semi-quantitative method. We confirmed 64 (18.1%) UTI cases by culture, the gold standard for UTI diagnosis. Fever was the most common clinical manifestation in UTI cases. The sensitivity, specificity, positive predictive value and negative predictive value of pyuria detected by microscopic urinalysis to identify UTI were 50%, 70.9%, 27.6% and 86.5% respectively. In 318 febrile cases, the sensitivity, specificity, positive predictive value and negative predictive value of pyuria detected by microscopic urinalysis to identify UTI were 73.2%, 72.6%, 28.3% and 94.8% respectively. The findings suggest pyuria detected by microscopic urinalysis as not a worth while marker of pediatric UTI. But it is a trust worthy marker in febrile pediatric cases.

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Abstract
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Published
2019-12-29
How to Cite
Shrestha, D., Thapa, P., Bhandari, D., Bhattachan, B., Parajuli, H., Chaudary, P., Sharma, V., & Shah, P. (2019). Detection of Pyuria by Microscopic Urinalysis as a Marker of Pediatric Urinary Tract Infection. Nepal Journal of Biotechnology, 7(1), 15-20. https://doi.org/10.3126/njb.v7i1.26946
Section
Original Research Articles