Antibiotic Susceptibility Pattern of Staphylococcus aureus Isolated from Pus/Wound Swab from Children Attending International Friendship Children's Hospital

Authors

  • Bidhya Maharjan St. Xavier's College, Tribhuvan University, Maitighar, Nepal https://orcid.org/0000-0002-9769-1201
  • Shovana Thapa Karki International Friendship Children's Hospital, Maharajgunj, Kathmandu, Nepal
  • Roshani Maharjan Tri-Chandra Multiple College, Tribhuvan University, Ghantaghar, Kathmandu, Nepal

DOI:

https://doi.org/10.3126/njb.v9i1.38645

Keywords:

Pus/wound swab, Staphylococcus aureus, Antibiotic susceptibility test (AST), Multidrug resistance (MDR), Methicillin resistance, MRSA, D-test

Abstract

A wound gets infected when the organism gets invaded through the breached skin, proliferated and production of various enzymes, toxins, etc. In order to treat the wound infection, antibiotic susceptibility pattern of organism should be determined before the prescription of the medicine. The present study was conducted from September 2017 to March 2018 with an aim to determine antibiotic susceptibility pattern of Staphylococcus aureus identified from the pus/wound swab among the patients visiting the International Friendship Children's Hospital, Kathmandu, Nepal. Total 270 sample were processed, isolated and identified using standard microbiological procedure and biochemical test. Antibiotic susceptibility test was carried out by using Modified Kirby Bauer's Disc Diffusion Method. Out of total sample, 51.48% (139) showed growth. The growth distribution was found to be high in out-patient department 84.9% (118) than in-patient department 15.1% (21). Among 139 positive growth, 83.5% were gram positive and 16.5% were gram negative. All together 12 different organisms were identified, among which S. aureus was found to be predominant organism 105 (75.5%). S. aureus was found to be sensitive towards Linezolid followed by Doxycycline whereas it was found resistant towards Ciprofloxacin. Among S. aureus identified, 50% were Multidrug resistant (MDR) S. aureus and 55% were Methicillin resistance S. aureus (MRSA). MRSA was found to be sensitive towards Linezolid followed by Doxycycline and resistant towards Ciprofloxacin. The association between MDR and MRSA was found positively significant (i.e. p-value = 0.000). All strains of S. aureus were found to be sensitive towards Vancomycin.  22.86% were double disk diffusion test (D-test) positive. The prevalence of D-test was found to be high in MRSA (75%). The relationship between D-test and MRSA was found to be significantly correlated with each other (r = 0.39). Linezolid, Chloramphenicol, Vancomycin and Doxycycline is a drug of a choice for both S. aureus and MRSA infection.

Downloads

Download data is not yet available.
Abstract
212
PDF
155

Author Biographies

Bidhya Maharjan, St. Xavier's College, Tribhuvan University, Maitighar, Nepal

Department of Microbiology

Shovana Thapa Karki, International Friendship Children's Hospital, Maharajgunj, Kathmandu, Nepal

Department of Pathology

Roshani Maharjan, Tri-Chandra Multiple College, Tribhuvan University, Ghantaghar, Kathmandu, Nepal

Department of Microbiology

Downloads

Published

2021-07-31

How to Cite

Maharjan, B., Karki, S. T., & Maharjan, R. (2021). Antibiotic Susceptibility Pattern of Staphylococcus aureus Isolated from Pus/Wound Swab from Children Attending International Friendship Children’s Hospital. Nepal Journal of Biotechnology, 9(1), 8–17. https://doi.org/10.3126/njb.v9i1.38645

Issue

Section

Original Research Articles