Potential surface active agent production using very low grade and cheap substrate by Bacillus subtilis as microbial cell factory

Authors

  • Niranjan Koirala Department of Natural Products Research, Dr. Koirala Research Institute for Biotechnology and Biodiversity, Kathmandu 44600, Nepal; Laboratory of Biotechnology, Faculty of Science and Technology, University of Macau, Macau SAR 999078, China
  • Sareeta Khanal Department of Natural Products Research, Dr. Koirala Research Institute for Biotechnology and Biodiversity, Kathmandu 44600, Nepal; Department of Microbiology, Central Campus Technology, Dharan 56700, Tribhuvan University, Nepal.
  • Sujan Chaudhary Department of Microbiology, Central Campus Technology, Dharan 56700, Tribhuvan University, Nepal; Department of Botany, Amrit Science Campus, Kathmandu 44600, Tribhuvan University, Nepal
  • Sagar Gautam Department of Microbiology, Trichandra Multiple Campus, Ghantaghar, Kathmandu 44600, Tribhuvan University, Nepal
  • Shiv Nandan Sah Department of Microbiology, Central Campus Technology, Dharan 56700, Tribhuvan University, Nepal
  • Prince Subba Department of Microbiology, Central Campus Technology, Dharan 56700, Tribhuvan University, Nepal
  • Najat Marraiki Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia
  • Gaber El-Saber Batiha Department of Pharmacology and Therapeutics, Faculty of Veterinary Medicine, Damanhour University, Damanhour 22511, AlBeheira, Egypt

Keywords:

Bacillus subtilis, Bio-surfactants, Emulsification index, Hydrocarbons, Surface tension

Abstract

Bio-surfactants are surface-active molecules which are produced by the wide range of microbes including bacteria, fungi, moulds, and yeast. This study was conducted to identify bio-surfactants by Bacillus subtilis combined with use of cheap substrates and industrial wastes (Mustard cake, Whey and Soya cake) which are found locally in Nepal. Bacillus subtilis, one of the most potential bio-surfactants producer; was isolated from soil sample of hydrocarbon contaminated site. Isolates were grown in a Minimal Salt Media (MSM) with 10% (v/v) mustard oil cake, whey and soya cake separately. The presence and potential of surfactant was determined by the oil spreading technique, emulsification index (%E24) and surface tension measurement. It was revealed that the surface tensions of cell free extract were 54.41, 60.02 and 56.64 mN/m for from mustard cake, whey and soya cake respectively as compared to distilled water (72.09) at 25oC. The emulsification index values was found to be highest in engine oil from the bio-surfactant extracted from mustard cake, soya cake and whey respectively. Similarly, mustard oil showed the lowest value of emulsification index. The highest emulsification activity was shown in mustard oil i.e. 1.13 from the cell free extract from mustard oil and lowest in engine oil i.e., 0.07, by the extract from soya cake medium, when measured in spectrophotometer at 540 nm. In conclusion, strain of Bacillus subtilis was found to be the potential surface active agent producers on the mustard oil cake, which can be useful medium for various environmental, food, medicinal and industrial processes.

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Published

2021-12-30

How to Cite

Koirala, N., Khanal, S., Chaudhary, S., Gautam, S., Sah, S. N., Subba, P., Marraiki, N., & Batiha, G. E.-S. (2021). Potential surface active agent production using very low grade and cheap substrate by Bacillus subtilis as microbial cell factory. Nepal Journal of Biotechnology, 9(2), 21–28. Retrieved from https://www.nepjol.info/index.php/NJB/article/view/41910

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Section

Original Research Articles